Structural and functional analyses of vesicular stomatitis virus P gene products.
Item
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Title
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Structural and functional analyses of vesicular stomatitis virus P gene products.
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Identifier
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AAI9605652
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identifier
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9605652
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Creator
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Richardson, James Cornelius.
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Contributor
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Adviser: Richard Peluso
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Microbiology | Biology, Molecular
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Abstract
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The growth of vesicular stomatitis virus requires two distinct RNA synthetic events: transcription of messenger RNA molecules, and replication of the viral genome RNA. This thesis reports the use of a panel of monoclonal antibodies directed against the viral phosphoprotein P in an attempt to assess the role of this protein in RNA synthesis. Using extracts derived from virus-infected cells, I show that several anti-P monoclonal antibodies have a specific inhibitory effect on genome RNA replication with minimal effects on mRNA transcription. I also show that the P protein to which one of these antibodies (6D11) is directed is not complexed with the N protein and that the amount of soluble P protein that binds to the 6D11 antibody in immunoprecipitation reactions can be increased by treating extracts with alkaline phosphatase. In addition, phosphatase treatment of infected cell extracts results in an increased level of genome RNA replication. These results suggest that a soluble sub-species of the P protein that functions in genome RNA replication exists in infected cells, and that this species of the P protein is not required for transcription. This thesis also reports the identification of two small basic proteins encoded by the P gene in an alternate reading frame. These proteins, which have not previously been shown to exist in VSV-Indiana, are called C and C{dollar}\sp\prime{dollar} and are shown to be present in infected cells and to be associated with ribonucleoprotein complexes.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
