Fluorescence image and spectrum in random media and cancer cells.
Item
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Title
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Fluorescence image and spectrum in random media and cancer cells.
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Identifier
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AAI9605688
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identifier
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9605688
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Creator
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Zang, Zhiwei.
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Contributor
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Adviser: Samir A. Ahmed
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Date
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1995
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Language
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English
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Publisher
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City University of New York.
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Subject
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Engineering, Electronics and Electrical | Engineering, Biomedical
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Abstract
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This thesis investigates (1) The capability of using a fluorescence-absorption technique to image objects hidden in scattering media, (2) The effect of multiple light scattering and self absorption on the fluorescence and excitation spectra of dye in random media, and (3) DNA ploidy measurements in breast and colon cancer using fluorescence image cytometry.;To see an object hidden in or behind a highly scattering random medium is one of the most challenging physics and engineering problems. Many different techniques have been introduced for this purpose. In this thesis we introduce an exceedingly simple, yet novel and important technique, fluorescence-absorption technique to improve our ability to see through a highly scattering random medium. Since a luminescent object to be detected in a typical medical application, such as a breast tumor is itself constituted from a highly scattering medium, the effect of scatter on the emitted luminescence spectra itself is of interest to the further development of these techniques. This is of particular importance since the distribution of the luminescent spectrum itself may relate to medical conditions to be diagnosed. We presents here the results of experiments to examine the impact of internal multiple scattering within a luminescent body on the luminescence spectrum observed at the surface.;New research and development opportunities and advances are evident in the multidisciplinary field of computer-aided microscopy for cell and tissue imaging and analysis. This growth applies to both the development of new and improved scientific method as well as to the utilization of these techniques to investigate significant biomedical applications. Much of the core and supporting technology in this work involves optical and visual process. We, with The Mount Sinai Hospital, measured DNA ploidy for the cancer cells diagnosis research.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
