Identification ofcis-acting regulatory sequences required for transcriptional control of theard gene of a neuronal nicotinic acetylcholine receptor from Drosophila.
Item
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Title
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Identification ofcis-acting regulatory sequences required for transcriptional control of theard gene of a neuronal nicotinic acetylcholine receptor from Drosophila.
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Identifier
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AAI9630526
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identifier
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9630526
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Creator
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Yang, Lee-Chuan.
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Contributor
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Adviser: Thomas Schmidt-Glenewinkel
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Date
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1996
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry
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Abstract
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As part of a long-term goal to elucidate the cellular and molecular mechanisms controlling the expression of neuronal nAChR subunit genes in Drosophila, the cis-acting regulatory region involved in the expression of the ard subunit gene has been identified and characterized. DNA fragments of the 5{dollar}\sp\prime{dollar} flanking region were fused to the Escherichia coli lacZ reporter gene and introduced into the Drosophila germ line by P-element-mediated transformation. A 325 bp fragment of the 5{dollar}\sp\prime{dollar} flanking sequence was sufficient to direct {dollar}\beta{dollar}-galactosidase expression in the nervous tissue during development with a pattern very similar to the distribution of transcripts of the corresponding ard gene. The results indicate that the ard gene lacks a TATA box and GC-rich sequence at the transcription start site and initiates transcription at a tightly clustered site.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
