Vibrational circular dichroism of gramicidin: Conformational studies in lipid bilayer and organic solvent.
Item
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Title
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Vibrational circular dichroism of gramicidin: Conformational studies in lipid bilayer and organic solvent.
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Identifier
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AAI9707062
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identifier
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9707062
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Creator
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Agbaje, Ismailu Olugbemiga.
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Contributor
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Adviser: Max Diem
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Date
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1996
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry
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Abstract
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In this dissertation we report the first vibrational circular dichroism of gramicidin (GA) in a model lipid bilayer, dimyristoylphosphatidylcholine (DMPC), as well as in an organic solvent (pyridine). Our findings reveal that gramicidin is inserted into the bilayer as an extended left-handed {dollar}\beta\sp{lcub}6.3{rcub}{dollar}-helix, which is believed to be the ion conducting dimer that is arranged in an N- to -N-termini conformation. In pyridine, gramicidin adopts a left-handed, intertwined double stranded {dollar}\beta\sp{lcub}5.6{rcub}{dollar}-helix. This may be a minor form of the membrane-bound ion conducting gramicidin channels. Our observations support several other spectroscopic conformational studies of GA.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
