Fourier transform infrared microspectroscopy of benign and neoplastic cervical squamous epithelium.
Item
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Title
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Fourier transform infrared microspectroscopy of benign and neoplastic cervical squamous epithelium.
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Identifier
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AAI9732900
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identifier
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9732900
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Creator
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Chiriboga, Luis Alberto.
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Contributor
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Adviser: Max Diem
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Date
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1997
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Biochemistry | Health Sciences, Pathology | Biophysics, Medical | Health Sciences, Oncology
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Abstract
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Fourier transform infrared spectroscopy is a well established analytical method that is extremely sensitive to molecular composition and structure. More recently, Fourier transform infrared spectrometers have been coupled with traditional light microscopes yielding an extremely powerful tool to observe and collect molecular level information from complex samples. In this study, Fourier transform infrared microspectroscopy (FT-IRM) is used to analyze formalin fixed, paraffin embedded, cervical tissue biopsies. The goal of this work is to identify and characterize the infrared spectral signatures of the various cellular components found in benign and neoplastic cervical squamous epithelium. The methods for obtaining the infrared spectra of formalin fixed, paraffin embedded tissue biopsies is described. The infrared spectra collected from various epithelial components of benign and neoplastic cervical epithelium are presented. These spectra are evaluated in terms of the major biochemical components present within cervical epithelial cells. The spectra are also related to the observed histopathology. The results show that FT-IRM is able to distinguish between the normal anatomic layers of benign cervical epithelium. These spectra correlate well with the normal biochemical composition of cervical epithelium. In neoplastic cervical epithelium, the spectra are significantly different. The observed spectral changes are consistent with the histopathology of neoplastic cervical epithelium. In addition, the morphologic changes seen in neoplastic cervical epithelium are mirrored by the infrared spectra. In conclusion, FT-IRM is capable of accurate and reproducible infrared measurement of precise anatomic locations based on correlation with observed histopathology in formalin fixed paraffin embedded tissue.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
