Characterization of a plasmid from the cyanobacterium Microcystis aeruginosa.

Title: Characterization of a plasmid from the cyanobacterium Microcystis aeruginosa.
Identifier: AAI9732983
identifier: 9732983
Creator: Wallace, Margaret M.
Contributor: Advisers: Shirley Raps | Thomas Schmidt-Glenewinkel
Date: 1997
Language: English
Publisher: City University of New York.
Subject: Biology, Molecular | Biology, Microbiology | Chemistry, Biochemistry
Abstract: The complete nucleotide sequence of the plasmid pMa025 from the unicellular, toxin producing cyanobacterium Microcystis aeruginosa UV025 was determined. The plasmid is 8018 bp in length and has a 62.3% G+C content.;Nineteen potential open reading frames (ORFs) were identified. The deduced amino acid sequence from a 1700 bp segment (5000-6700) of pMa025 was significantly similar to three putative cyanobacterial plasmid replication proteins. Within this region ORF M encodes a putative plasmid replication protein, RepA, that is significantly similar to a hypothetical plasmid replication protein from Synechococcus sp. strain PCC7942 plasmid pUH24.;Several regions of pMa025 showed significant similarity to putative genes and hypothetical proteins involved in bacterial polyketide and fatty acid synthesis. pMa025 may be involved in the biosynthesis of secondary metabolites produced by Microcystis.;A gene-transfer system, based on the shuttle vector pMaLIND7, was developed.
Type: dissertation
Source: PQT Legacy CUNY.xlsx
degree: Ph.D.

Media: Characterization of a plasmid from the cyanobacterium Microcystis aeruginosa.