Micellar electrokinetic capillary chromatography with single and double alkyl chain cationic surfactants.
Item
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Title
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Micellar electrokinetic capillary chromatography with single and double alkyl chain cationic surfactants.
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Identifier
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AAI9807912
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identifier
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9807912
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Creator
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Chen, Kangmin.
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Contributor
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Adviser: David C. Locke
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Date
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1997
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Language
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English
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Publisher
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City University of New York.
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Subject
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Chemistry, Analytical
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Abstract
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Micellar electrokinetic capillary chromatography (MECC) with single and double alkyl chain cationic surfactants was investigated in four different projects.;First, the separation of the eleven priority pollutant phenols was investigated with a cationic surfactant (C{dollar}\sb{16}{dollar}TAB) in MECC. The effects of buffer constituents, acetonitrile (ACN) additive, pH, ionic strength and surfactant concentration in the background electrolytes (BGEs) on retention behaviour were studied in detail. The most important factor in the separation was the amount of ACN in the BGEs.;Second, the interaction between charged and uncharged solutes and C{dollar}\sb{16}{dollar}TAB was investigated in MECC. For ionized acidic solutes with increasing ionic strength of the BGE, the net mobilities increased and the capacity factors decreased; however, for cationic, uncharged and slightly ionized acidic solutes, the net mobilities and the capacity factors were almost not affected. A fundamental theoretical and applied study of net mobility, capacity factor and distribution coefficient as a function of ionic strength in the BGEs was carried out.;Third, the use of a series of novel double alkyl chain (Gemini) cationic surfactants (Gemini-C8 to -C18) was evaluated in MECC. For comparison, the single chain surfactants (C{dollar}\sb{12}{dollar}TAB, C{dollar}\sb{14}{dollar}TAB, and C{dollar}\sb{16}{dollar}TAB) were examined as well. With increasing alkyl chains and concentration of each surfactant, the capacity factors increased linearly, but the net mobilities of both model solutes and the micelles changed differently and depended upon the surfactant concentrations. The best separation of the eleven priority pollutant phenols was achieved using Gemini-C12.;Last, two Gemini surfactants (Gemini-C12 and -C14) were synthesized and used in MECC for indicating purity and stability of dihydroergotoxine mesylates, therapeutic substances for the treatment of dysregulation of the circulatory system. For comparison, C{dollar}\sb{14}{dollar}TAB and C{dollar}\sb{16}{dollar}TAB were also utilized in MECC. Seventeen ergot alkaloids were well resolved in eight minutes using Gemini-C12 as the micelle-forming reagent; and Gemini-C14 provided better resolution for all the alkaloid isomers. These MECC separations could not be achieved using the single chain surfactants.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
