A genetic screen for developmentally regulated genes in mice.
Item
-
Title
-
A genetic screen for developmentally regulated genes in mice.
-
Identifier
-
AAI9820510
-
identifier
-
9820510
-
Creator
-
Battaglino, Ricardo Anibal.
-
Contributor
-
Adviser: Heidi Stuhlmann
-
Date
-
1998
-
Language
-
English
-
Publisher
-
City University of New York.
-
Subject
-
Biology, Genetics | Biology, Molecular
-
Abstract
-
This project was aimed at the identification of developmentally regulated genes in mice. The early mid-gestation stage of development (E6.0 to E9.0) is particularly important because a number of complex events take place at that time (gastrulation, onset of organogenesis, establishment of the A-P axis) which will set the basic body plan and affect the subsequent development of the embryo. Identification of genes with a possible role in the aforementioned processes will increase our understanding of mouse development. I designed a promoter-trap strategy to screen for genes that are regulated during early-mid gestation. Mouse ES cells were infected with promoter-trap retroviral vectors that carry a promoter-less human alkaline phosphatase (PLAP) reporter gene. Infected ES cells were induced to differentiate in vitro into embryoid bodies and clones were selected on the basis of their differential expression of the PLAP reporter gene. The rationale to this approach assumes that a number of cellular and molecular events that take place during the differentiation of ES cells into embryoid bodies recapitulate events that occur in vivo during early post-implantation development. One ES clone, promoter trap clone IVE38, was isolated and tested for expression of the PLAP reporter gene during embryogenesis. To this end, chimeric embryos were generated by aggregating E38 ES cells with tetraploid embryos. PLAP reporter gene expression was found to localize, mainly, to the paraxial (somitic) mesoderm in E9.0 embryos. Expression was also observed in the neural tube PLAP expression data suggests a role for the trapped endogenous locus in somitic mesoderm differentiation.
-
Type
-
dissertation
-
Source
-
PQT Legacy CUNY.xlsx
-
degree
-
Ph.D.
