Characterization of upstream sequences required for transcriptional control of the als gene encoding a subunit of a neuronal nicotinic acetylcholine receptor from Drosophila.
Item
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Title
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Characterization of upstream sequences required for transcriptional control of the als gene encoding a subunit of a neuronal nicotinic acetylcholine receptor from Drosophila.
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Identifier
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AAI3144117
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identifier
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3144117
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Creator
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Ma, Dongdong.
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Contributor
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Adviser: Thomas Schmidt-Glenewinkel
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Date
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2004
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Neuroscience
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Abstract
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The als gene (nAcRalpha-96Aa) encodes an alpha-like subunit of a neuronal nicotinic acetylcholine receptor (nAChR) from Drosophila. To understand how als expression is regulated, we report here the molecular characterization of the als gene and in vivo analysis of upstream regulatory sequences which confer the correct temporal and spatial expression. Primer Extension and RACE-PCR analysis in both embryo and adult flies allowed us to establish the transcription start site of als. Upstream fragments of 2.9 kb and 1.25 kb including the transcription start site and partial noncoding exon were cloned into the P-element vector pCaSpeR-AUG-betagal generating promoter fusions with lacZ reporter gene and used to transform Drosophila. Both upstream fragments were shown to drive als expression in the central nervous system and reproduce the tissue and developmental expression pattern as comparable to that of the endogenous gene als (in embryo) and ard (in adult).
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
