Molecular systematics of the Repleta species group of the genus Drosophila.
Item
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Title
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Molecular systematics of the Repleta species group of the genus Drosophila.
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Identifier
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AAI9986322
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identifier
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9986322
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Creator
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Durando, Celestine M.
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Contributor
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Adviser: Marvin Wasserman
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Date
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2000
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Language
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English
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Publisher
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City University of New York.
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Subject
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Biology, Molecular | Biology, Genetics
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Abstract
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The species in the repleta group of the genus Drosophila have been placed into S subgroups---the mulleri, hydei, mercatorum, repleta and fasciola subgroups. Each subgroup has been further subdivided into complexes and clusters. Fifty-four taxa, including 46 taxa belonging to the repleta species group were sequenced for fragments of 4 genes---16S ribosomal DNA (16S), Cytochrome oxidase II (CO II) and Nitrogen dehydrogenase1 (ND1) of the mitochondrial genome, and a region of the hunchback (hb) nuclear gene. We also generated a partial data set of Elongation factor 1-alpha (Ef1alpha) sequences for a subset of taxa. Our analysis used both DNA characters and chromosomal inversion data. The phylogenetic hypothesis we obtained supports many of the traditionally accepted clades within the mulleri subgroup, but the monophyly of taxonomic groups outside of this subgroup appears not to be supported. Phylogenetic analysis revealed one well supported, highly resolved clade that consists of closely related members of the mulleri and buzzatii complexes.;We placed the classical Drosophila evolutionary biology tools of polytene chromosome inversion analysis and crossability tests in a molecular phylogenetic context. A simultaneous analysis framework was used to examine the congruence of the chromosomal inversion data with more recent DNA sequence data in four Drosophila species groups---the melanogaster, the virilis, the repleta and the Hawaiian Drosophila species groups. Crossability data can also be examined in a tree based context in these four species groups by examining phylogenetic distances that crossability can traverse. The comparisons we made allowed us to detect aspects of crossability such as the importance of geographic affinity (sympatry and allopatry) that are characteristic of the species groups we included in this study.;We investigated the species belonging to the Drosophila mayaguana subcluster at the level of the population using DNA sequence data. We generated phylogenetic trees, performed Population Aggregation Analysis and examined the group from a population genetics perspective.
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Type
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dissertation
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Source
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PQT Legacy CUNY.xlsx
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degree
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Ph.D.
